mouse inflammation antibody array  (Danaher Inc)

Journal: Scientific Reports

Article Title: Neuroimmune characterization of optineurin insufficiency mouse model during ageing

doi: 10.1038/s41598-023-38875-3

Figure Lengend Snippet: Similar inflammatory profiles in the brains and spinal cords of aged Optn 470T and WT mice. RayBio ® C-Series Mouse Inflammation Antibody Array C1 was performed on brain and spinal cord lysates from two-year-old male WT and Optn 470T mice. Selected pro- and anti-inflammatory cytokines in the brain ( A ) and spinal cord ( D ) from WT and Optn 470T mice are shown normalized to WT. Three independent experiments were performed from the following groups of mice: (1) 4 WT and 5 Optn 470T ; (2) 1 WT, 5 Optn 470T ; (3) 4 WT, 3 Optn 470T . Brain lysates from 9 two-year-old male WT and 13 Optn 470T mice were assayed by ELISA for TNF-α ( B ) and IFN-β ( C ). The data were analysed by Mann–Whitney (( A ): ITAC, IL-4; ( C,D ): IL-4) and Student’s t -test (( A ): TNF-α, CCL2, fractalkine; ( B,D ): XCL1, TNF-α, CCL2, fractalkine); *p < 0.05.

Article Snippet: Pierce BCA Protein Assay Kit (#23227) was from Thermo Scientific (Waltham, MA, USA), and RayBio ® C-Series Mouse Inflammation Antibody Array C1 kit (#126AAM-INF-1-8) from Raybiotech (Norcross, GA, USA).

Techniques: Ab Array, Enzyme-linked Immunosorbent Assay, MANN-WHITNEY